Finally, 1 μCi of 86Rb was included to every lifestyle and incubation was continued at 37 °C for an extra ten min. Reactions were terminated by washing the cells with ice-cold 100 mM MgCl2 and cell lysates were well prepared for liquid scintillation counting. The 86Rb counts in parallel cultures https://edwardn530glo3.wikitelevisions.com/user