Nicking reaction catalysed with the MobA89K and MobC89K proteins. The cleaved strand is then unwound with the helicase and shipped to the 89K-encoded GI-form T4SS machinery. The VirB1-89K part is believed to act as a lytic transglycosylase, locally opening the peptidoglycan with contributions from some other component(s) over the assembly https://89ke-info98642.azzablog.com/29904721/89k-for-dummies